Інститут біохімії ім. О.В Палладіна НАН України

Palladin Institute of Biochemistry of the NAS of Ukraine

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Department of Molecular Biology


Head – Oleksandr G. Minchenko,
Dr. Sc.
Tel.: + (380-44) 235-31-37
CURRENT PROJECTS:

  1. “Molecular mechanisms of the regulation of gene expression in normal and pathologic conditions”.
  2. “Molecular mechanisms of the regulation of transcription factor gene expression”.
  3. Ukrainian-Japan collaboration: “Expression hypoxia responsive genes in malignant tumors”.



Prof. Oleksandr Minchenko and director Research center for Innovative Oncology, president National
Cancer Center of Japan, prof. Hiroyasu Esumi in the Research center for Innovative Oncology laboratory, 24.06.2004.
4,Ukrainian-French Program “Dnipro”: “Role of the regulatory enzyme PFKFB in the regulation of tumor growth and
neovascularization: evaluation of the different isozymes as therapeutic targets for antitumor drugs” (“Role de l'enzyme PFKFB dans la regulation de la croissance tumorale et de la neovascularisation: evaluation des differentes isozymes en tant que cibles therapeutiques d'agents antitumoraux.”)



Prof. Oleksandr Minchenko and prof. INSERM 920 Laboratory of Molecular Mechanismsof Angiogenesis University Bordeaux1, France, 19.10.2007, in O.Palladin museum, Kyiv.
Selected publications:

  1. Klimes I., Sebokova E., Minchenko A., Maassen T., Moss R., Mitkova A., Wiersma M., Bohov P.: Glut4 gene expression and Glut4 protein levels in muscle of high sucrose-fat rats: effect of dietary fish oil. J. Nutr. Biochem. 5: 389-396, 1994
  2. Minchenko A., Bauer T., Salceda S., Caro J.: Hypoxic stimulation of vascular endothelial growth factor expression in vitro and in vivo. Laboratory Invest. 71: 374-379, 1994
  3. Aversa C.R., Oparil S., Caro J., Li H., Sun S.-D., Chen Y.-F., Swerdel M.R., Monticello T.M., Durham S.K., Minchenko A., Lira S.A., Webb M.L.: Hypoxia stimulates human preproendothelin-1 promoter activity in transgenic mice. Amer J. Physiol. 273 (Lung Cell. Mol. Physiol. 17), N 4: L848-L855, 1997
  4. Armstead V.E., Minchenko A.G., Schuhl R.A., Lefer A.M.: Regulation of P-selectin in human endothelial cells by nitric oxide. Amer. J. Physiol. 273: H740-H746, 1997
  5. Armstead V.E., Minchenko A.G., Campbell B., Lefer A.M.: P-selectin is up-regulated in vital organs during murine traumatic shock. FASEB J. 11, N 11: 1271-1279, 1997
  6. Scalia R., Hayward R., Armstead V.E., Minchenko A.G., Lefer A.M.: Effect of recombinant soluble P-selectin glycoprotein ligand-1 on leukocyte-endothelium interaction in vivo: role in rat traumatic shock. Circ. Res. 84: 93-102, 1999
  7. Scalia R., Armstead V.E., Minchenko A.G., Lefer A.M.: Essential role of P-selectin in the initiation of the inflamatory response induced by hemorrhage and reinfusion. J. Exp. Med. 189: 931-938, 1999
  8. Armstead V.E., Opentanova I.L., Minchenko A.G., Lefer A.M.: Tissue factor gene expression in vital organs during murine traumatic shock: role of transcription factors AP-1 and NF-?B. Anesthesiology 91: 1844-1852, 1999
  9. Minchenko A.G., Caro J.: Regulation of endothelin-1 gene expression in human microvascular endothelial cells by hypoxia and cobalt: role of hypoxia responsible element. Mol. Cell. Biochem 208: 53-62, 2000
  10. Mueller M.D., Vigne J.-L., Minchenko A.G., LebovicD.I., Leitman D.C., Taylor R.N.: Regulation of vascular endothelial growth factor (VEGF) gene transcription by estrogen receptors “?” and “?”. Proc. Natl. Acad. Sci. USA 97: 10972-10977, 2000
  11. Srinivas V., Leshchinsky I., Sang N., King M.P., Minchenko A., Caro J.: Oxygen sensing and HIF-1 activation does not require an active mitochondrial respiratory chain electron-transfer pathway. J. Biol. Chem., 2001, vol. 276: 21995-21998.
  12. Minchenko A.G., Leshchinsky I., Opentanova I., Sang N., Srinivas V., Armstead V.E., Caro J.: Hypoxia-inducible factor-1-mediated expression of the 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase-3 (PFKFB3) gene. Its possible role in the Warburg effect. J. Biol. Chem., 2002, vol. 277: 6183-6187.
  13. Minchenko O., Opentanova I., Caro J.: Hypoxic regulation of the 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene family (PFKFB-1-4) expression in vivo. FEBS Lett., 2003, vol. 554, p. 264-270.
  14. Minchenko A.G., Stevens M.J., White L., Abatan O.I., Komjati K., Pacher P., Szabo C., Obrosova I.G.: Diabetes-induced overexpression of endothelin-1 and endothelin receptors in the rat renal cortex is mediated via poly(ADP-ribose)polymerase activation. FASEB J., 2003, vol. 17, p. 1514-1516.
  15. Lanza-Jacoby S., Miller S., Jacob S., Heumann D., Minchenko A., Flynn J.: Hyperlipoproteinemic low-density protein receptor-deficient mice are more susceptible to sepsis than corresponding wild-type mice. J. Endotoxin Res., 2003, vol. 9, p. 341-347.
  16. Minchenko O.H., Opentanova I.L., Minchenko D.O., Ogura T., Esumi H.: Hypoxia induces transcription of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 gene via hypoxia-inducible factor-1alpha activation. FEBS Lett., 2004, vol. 576, 14-20.
  17. Minchenko O.H., Ochiai A., Opentanova I.L., Ogura T., Minchenko D.O., Caro J., Komisarenko S.V., Esumi H.: Overexpression of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-4 in the human breast and colon malignant tumors. Biochimie, vol. 87, N 11, 1005-1010, 2005.
  18. Minchenko O.H., Opentanova I.L., Ogura T., Minchenko D.O., Komisarenko S.V., Caro J., Esumi H.: Expression and hypoxia-responsiveness of the 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 in the mammary gland malignant cell lines. Acta Biochim. Pol., vol. 52, N 4, 881-888, 2005.
  19. Minchenko O.H., Ogura T., Opentanova I.L., Minchenko D.O., Esumi H.: Splice isoform of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-4: Expression and hypoxic regulation. Mol. Cell. Biochem., vol. 280, 227-234, 2005.
  20. Minchenko O.H., Ogura T., Opentanova I.L., Minchenko D.O., Ochiai A., Caro J., Komisarenko S.V., Esumi H.: 6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene family overexpression in the lung tumor. Укр. біохім. ж., 2005, 77, N 6, 46-50.
  21. Бобарикіна А.Ю., Мінченко Д.О., Опентанова І.Л., Ковтун О.О., Комісаренко С.В., Есумі Г., Мінченко О.Г.: Експресія мРНК HIF-1?, HIF-2? та VHL у різних лініях клітин при гіпоксії. Укр. біохім. журн., 2006, т. 78, № 2, 49 – 59. Bobarykina A.Y., Minchenko D.O., Opentanova I.L., Kovtun O.O., Komisarenko S.V., Esumi H., Minchenko O.H.: HIF-1?, HIF-2? and VHL mRNA Expression in Different Cell Lines under Hypoxia. Ukr. Biokhim. Zh., 2006, 78, N 2, 49 – 59.
  22. Bochkov V.N., Philippova M., Oskolkova O., Kadl A., Furnkranz A., Karabeg E., Breuss J., Minchenko O.H., Mechtcheriakova D., Hohensinner P., Rychli K., Wojta J., Resink T., Binder B.R., Leitinger N.: Oxidized phospholipids stimulate angiogenesis via induction of VEGF, IL-8, COX-2 and ADAMTS-1 metalloprotease, implicating a novel role for lipid oxidation in progression and destabilization of atherosclerotic lesions. Circ. Res., 2006. vol. 99, N 8: 900-908.
  23. Kovtun A.A., Minchenko A.G., Gudkov A.T. Mutational analysis of functional role of amino acid residues in domain IV of elongation factor G. Mol. Biol. (Mosk.), 2006, 40,?N 5, 850-856.
  24. Bobarykina A.Y., Minchenko D.O., Opentanova I.L., Moenner M., Caro J., Esumi H., Minchenko O.H.: Hypoxic regulation of PFKFB-3 and PFKFB-4 gene expression in gastric and pancreatic cancer cell lines and expression of PFKFB genes in gastric cancers. Acta Biochim. Pol., 2006, vol. 53(4), 789-799.
  25. Мінченко О.Г. Дивовижний світ бактерій і молекулярна біологія. Країна знань. 2007, № 1, 26-29.
  26. Mykhalchenko V.G., Minchenko D.O., Tsuchihara K., Moenner M., Komisarenko S.V., Bikfalvi A., Esumi H., Minchenko O.H. Expression of mouse 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3 mRNA alternative splice variants in hypoxia // Укр. біохім. ж. – 2008. – 80, № 1. – С. 19 – 25.
  27. Михальченко В.Г., Тсучігара K., Мінченко Д.O., Есумі Г., Приступюк O.M., Мінченко O.Г. Експресія мРНК 6-фосфофрукто-2-кінази/фруктозо-2,6-бісфосфатази-3 у щурів із стрептозотоциновим діабетом // Біополімери і клітина. – 2008. – 24, № 3. – С. 260 – 266.
  28. Minchenko D.O., Tsuchihara K., Komisarenko S.V., Moenner M., Bikfalvi A., Esumi H., Minchenko O.H. Unique alternative splice variants of mouse PFKFB-3 mRNA: tissue specific expression. Науковий вісник Національного медичного університету імені О.О.Богомольця, 2008, № 1, 72-78. Scientific Bulletin National O.O. Bohomoletz Medical University, 2008, № 1, 72-78.

BOOKS

  1. Minchenko A.G., Dudareva N.A.: Mitochondrial genome. NAUKA, Novosibirsk 1990, p. 1-194 (in Russian)
  2. Komissarenko V.P., Minchenko A.G., Tron'ko N.D.: Molecular mechanisms of Steroid Hormones Action. ZDOROVJA, Kyiv 1986, p. 1-192 (in Russian)


Staff of the Department of Molecular Biology:
Minchenko O.H., DSc, Head of department
Lisnyak I.O., DSc, leading researcher
Starodub M.F., DSc, main researcher
Nazarenko V.I., PhD, leading researcher
Kotsuruba A.V., PhD, senior researcher
Ivashkevych S.P., researcher
Kanjuk M.I., researcher
Bobarykina A.Y., researcher
Gordienko H.V., researcher
Gojster O.S., research assistant
Mykhalchenko V.G., research assistant
Kovtun O.O., research assistant
Terletsky B.M., research assistant
Marunych R.Y., research assistant
Korkach Y.P., research assistant

                  German-Ukrainian Symposium of Nanoscience and Nanotechnology 2008                 International Union of Biochemistry and Molecular Biology                        X biochemical UKRAINIAN the Congress September 13-17, 2010, Odessa.                     Ukrainian festival of science.                    National Academy of Sciences of Ukraine                 Scientific conferences, congresses, seminars, reading