Department of Muscle Biochemistry
Head – Sergiy O. Kosterin,
Dr. Sc., Corresponding Member of NASU, Prof.
Tel.:+ (380-44) 234-16-53
Research theme “Studying of biochemical mechanisms of regulation of Са2+ concentration in smooth muscle cells” The purpose of research – defining of biochemical mechanisms of the electro- and pharmaco-mechanical coupling in smooth muscles (mainly – myometrium, uterine smooth muscle, and other smooth muscles). Research focuses on comparative studying of energy-dependent Ca2+- and Na+-transporting systems of subcellular membrane structures. In particular, we are studing kinetic, catalitic and energetic properties of the plasma membrane calcium and sodium pumps, sarcoplasmic reticulum calcium pump and mitochondrial Ca2+ uniporter and regulation of their activity by physiologicaly active and pharmacological substances. Research theme “The structure-functional protein analysis at norm and some pathologies” The purpose of research – using of ATP-dependent enzyme proteins as models for investigation of effects of physiologicaly active and pharmacological substances on the intracellular cation exchange and contraction-relaxation cycle in smooth muscle. We are studying the Ca2+-transporting and contractile proteins and regulation of their activity by polyamines. Research theme “Research of influence and mechanisms of calixaren action on ionic transport systems in subcellular membrane structures of smooth muscles. The purpose of research – studing of biochemical mechanisms of calixaren action on energy-dependent Ca2+- and Na+-transporting systems of subcellular membrane structures (plasma membrane, sarcoplasmic reticulum, mitochondria) and on non-enzymatic ATP hydrolise. We are working in collaboration with sientists of Phosphoranes Chemistry Department, Institute of Organic Chemistry (head of the department – Dr.Sc., Prof., Corresponding Member of NASU V.I.Kalchenko) Selected publications
1. Kosterin S. Kinetics and energetics of Mg2+,ATP-dependent Ca2+ transport in the plasma membrane of smooth muscles. Neurophysiology. 2003, V.35, N3/4, P.215-228.
2. Sanborn B.M., Ku C-Y., Shlykov S.G., Babich L.G. Molecular Signaling Through G-Protein Coupled Receptors and the Control of Intracellular Calcium in Myometrium. J. Soc. Gynecol. Investig. 2005, V.12, N7, P.479-487.
3. Rodik R., BoikoV., Danylyuk O., Suwinska K., Tsymbal I, Slinchenko N., Babich L., Shlykov S., Kosterin S., Lipkowski J. and Kalchenko V. Calix[4]arenesulfonylamidines. Synthesis, structure and influence on Mg2+,ATP-dependent calcium pumps.
Tetrahedron Letters. 2005, V.46, N43, P.7459-7462.
4. Костерін С.О., Бабіч Л.Г., Шликов С.Г., Тітус О.В. та Борисова Л.А. Кінетична модель впливу сперміну на Mg2+,АТР-залежний транспорт іонів Са у мітохондріях гладенького м’яза. Укр. біохім. журн. 2005, Т.77, №3, С.76-86.
5. Vadziuk O., Pivovarenko V. and Kosterin S. Fluorometric detection of adenosine triphosphate with 3-hydroxy-4’-(dimethylamino)flavone in aqueous solutions. Journal of Fluorescence. 2006, V.16, N1, P.9-159.
6. Yushchenko D.A., Vadzyuk O.B., Kosterin S.O., Duportail G., Mely Y., Pivovarenko V.G. Sensing of adenosine-5-triphosphate anion in aqueous solutions and mitochondria by a fluorescent 3-hydroxyflavone dye. Analytical Biochemistry.
2007,V369, P.218-225.
7. Klyachina M.А., Boyko V.І., Yakovenko A.V., Babich L.G., Shlykov S.G., Kosterin S.О., Khilya V.P., Kalchenko V.I. Calix[4]arene N-Сhalconeamides: Synthesis and Influence on Mg2+,АТP-Dependent Са2+ Accumulation in the
Smooth Muscle Subcellular Structures J. Inclusion Phenomena and Macrocyclic Chemistry. 2008, V60, N1-2, P.131-137.
8. Векліч Т.О., Шкрабак О.А., Костерін С.О. Вплив сперміну на кінетичні параметри Nа+,К+-залежного та Mg2+-залежного гідролізу АТР у плазматичній мембрані міоцитів матки. Укр. біохім. журн. 2008.
