Department of Metabolism Regulation
Head – Mykola Dmytrenko,
Dr.Sc., Prof.
Tel.:+ (380-44) 234-43-45
Current project:
1. Metabolic pathways, metabolic pathway relations and their regulation mechanisms under normal and pathological conditions in vivo The role of formaldehyde and nitrogen oxide interaction in the mechanism of their biologic action. Investigation of some key-note reactions that combine NO and formaldehyde metabolism and the presence of correlate interactions between these reactions and oxidant-antioxidant system. For this work we use the experimental models, in which hyper production of the formaldehyde and NO in rats is depicted. The role of the formaldehyde and NO interaction in the pathogenesis of experimental lathyrism, diabetes etc. We give special attention to the detection of changes in the free formaldehyde and NO levels, to the research of correlative interactions in the NO and formaldehyde metabolisms and to the investigation of the prooxidant-antioxidant processes in the cells and tissues of the organism, to the study of changes in collagen and other out-matrix macromolecular substances of the musculoskeletal system. The character of cytotoxic action of donors and acceptors of formaldehyde and NO is studied. Investigation of the role of stomach helicobacteriosis in the manifestation of side-effects after application of some drugs and toxicants. Examination of the possibility of alternative ways of the methanol toxic action.
2. The improvement of the criteria-significant biochemical indicators and the methodological basics of their use for the assessment of metabolism condition.Creation of integral criteria and separate biochemical tests for the evaluation of the influence on the organism by such factors as the pathological conditions, the chemicals and physical factors. Development of standard operational procedures according to the international requirements. Searching for the biomarkers that identify the charge of formaldehyde and the charge of nitrogen oxide on the organism, also the investigation of the effective methods and instruments for the prophylaxis and cure the diseases, and for decrease of side effects at the therapy. Development of new dietary additives and pharmaceutical agents that can influence on the formaldehyde and NO levels, and counteract their harmful effect on the organism. This work is made on the basis of the new data of metabolic ways of NO and formaldehyde interaction and their common toxic action on the organism.
Department staff
M.P. Dmytrenko, doctor of Biology, Professor, Head of department
L.V. Gudkova, PhD, Senior Scientist
T.T. Volodina, PhD, Senior Scientist
T.O. Kishko , PhD, Senior Scientist
L.M. Petrun, PhD, Senior Scientist
T.M. Pechenova, PhD, Senior Scientist
N.V. Silonova, PhD, Senior Scientist
V.V. Sushkova, PhD, Senior Scientist
N.D. Dzvonkevich, PhD, Scientist
N.V. Latishko, PhD, Scientist
S.G. Shandrenko, PhD, Scientist
L.G. Kapustyanenko, Junior Scientist
V.I. Ovcharenko, Junior Scientist
N.F. Shevtsova, Junior Scientist
O.O. Gudkova, Senior Engineer
N.M Kasyanova, Senior Engineer
N.M.Popova, Senior Engineer
L.V. Tsyakalo, Senior Engineer
List of basic publications (recent 5 years)
1. N.P. Dmitrenko, A. Holian. Role of the paths of formaldehyde and nitric oxide metabolism interdependence in mechanism of their toxic action. I. exo- and endogenous sources of formaldehyde and nitric oxide. Toxic action of formaldehyde // Ukr. Biochem. Journ. – 2005. - 77, № 1.-p.-38-47 (Reviews)
2. N. P. Dmitrenko, A. Holian. Role of interaction of metabolism paths of formaldehyde and nitrogen oxide in the mechanism of their toxic effect.
2. Toxic effect of nitrogen oxide.// Ukr. Biochem. Journ. .- 2005.-77, № 4.- p 5-26. (review)
3. M.F. Guly, N.V. Silonova, T.M. Pechenova, N.F. Shevtsova, L.P. Klimenko. Influence of formiate on freeamino acids contents in tissues and organs of rats in vitro and in vivo // Ukr. Biochem. Journ. 2005.-1.-p. 41-47
4. M.P. Dmitrenko, V.M. Mihailenko, A.A. Glavin et al. Metabolism of n-nitrosodimethylamine in rats with diabetes induced by streptozotocine // Modern Problems of Toxicology .-2006-N 4.-p.-21-26
5. G.M. Prodanchuk, S.G. Shandrenko, T.O. Kishko, N.P. Dmitrenko. New aspects of hydroxylamine toxic action mechanism // Modern Problems of Toxicology .- 2006, N 1.-p.-37-46
6. Nely Latyshko , Ludmyla Gudkova ,Olga Gudkova , Volodymyr Mykhailovsky . Molecular mechanisms of catalase action under stress conditions. // Annales Universitatis Mariae Curie-Skladowska, Lublin - Polonia. 2006, Vol. XIX, N1, 32, P 159-162
7. N. P. Dmitrenko, A. Holian. Role of interaction of metabolism paths of formaldehyde and nitrogen oxide in the mechanism of their toxic effect. 3. Main metabolism sites of formaldehyde and nitrogen oxide mediating their effect. //Ukr. Biochem. Journ. – 2007 .-79, № 5, pp. 65-80. (review)
8. Volodina T.T., Pechenova T.M., Dzvonkevich N.D., Popova N.М., Silonova N.V.,Astahova V.S., Panchenko L.М.,Guly M.F., Mikhaylovsky V.О. Modifying effect of lowmolecular metabolities on the state of extracellular matrix of connective tissue of animals.// Ukr. Biochem. Journ. 2007, N6., p.52-65.
9. M. F. Guly, N.V. Silonova, T.M. Pechenova, N.F. Shevtsova. About the possibility of reverse formylation of amino acids in rat tissues at investigations in vivo and in vitro. // Reports of National Academy of Sciences of Ukraine, 2007, N 3, p.- 36-38
10. T.O. Kishko, S.G. Shandrenko, N.P. Dmitrenko. Arginine: biological action, influence on synthesis of nitrogen oxide.// Journ. of Academy of Medical Sciences of Ukraine - 2008, № 1.- p
