Department of Enzyme Chemistry and Biochemistry
Head – Stanislav O. Kudinov,
Dr. Sc., Prof.
Tel.:+ (380-44) 234-90-56
Department of Enzyme Chemistry and Biochemistry The name of subjects/ projects and their short contents Research project “The study of fibrinolysis mechanism at the normal state, acute phase and oxidative stress conditions as a base for new plasminogen-containing class of thrombolytic drugs production“. The name of the program: Departmental NASU
The field of research is:
• discoordination in fibrinolytic cascade due to abnormal levels of plasminogen, tissue plasminogen activator(t-PA), plasminogen activator inhibitor (PAI-1) levels and the level of protein C in the blood plasma of the patients with thrombogenic pathologies;
• causal relationships between inflammation and coagulation/fibrinolysis;
• detection of redox-sensitive proteins in fibrinolytic system and experimental approaches to their involvement in redox modification process.
Research project: „Investigation of the molecular mechanism of fibrinolysis in the state of acute phase accompanying thrombogenic diseases”. The program :“ The modern medico-biologic problems and human environment”.
The field of research is:
• The study of fibrinolysis mechanism under conditions of protein C deficiency.
• The investigation of cause and effect relationship between the appearance of acquired protein C deficiency and the development of inflammation or oxidative stress.≠
• The elaboration of the methods for testing of protein C level and functional activity in the blood protein preparations.
In the frame of research project (N15) “Structural and functional analysis of the proteins at the normal and pathological state”: Chapter “ The elaboration of the method for obtaining of protein C concentrate.”The complex purposeful program “
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The investigations directed on:
• The elaboration of the methods of synthesis of metal- affinitive sorbents for protein C purification from human plasma on the base of copolymerization of allyl and methacrylate monomers with acrylamide.
• The elaboration of methods for protein C purification from Cohn fractions and the testing of quality of the obtained final product.
Scientific stuff of the Department and List of thear publications:
The chairman of the Department - doctor of biological science, prof. Kudinov Stanislav Olexandrovich. Prof. Kudinov S.O. has been investigating the fibrinolysis mechanism since 1980 th. He particularly investigates the structure of plasminogen molecule, as well as the localization, chemical nature and specificity of its interaction with substrates. He is the author of conception about the physiological relevance of thrombolytic therapy by plasminogen preparation. His present scientific interests concern the investigation of pathophysiological mechanism of fibrinolysis and the ways of its correction.
Some publications:
1. Kastrukina G.F., Taran L.D., Kudinov S.A. Kinetic characteristics of fibrinogen and fibrin hydrolysis by plasmin 1 and 2 and miniplasminogen. / Thromb. Res. - 1986. - V. 41, №5. - P. 681-688.
2. Verevka S.V., Kudinov S.A.,Grinenko T.V. Arginyl-binding sites of human plasminogen. Thrombos. Res. 1986. V.41. p.689-698.
3. Lezhen T.I., Kudinov S.A., Medved’ L.V. Plasminogen-binding site of the thermostabke region of fibrinogen fragment D. Febs. Letters. March 1986. V 197. Num.1,2. P. 59-62.
4. Novokhatny V.V., Matsuka Y .V., Kudinov S.A. Analysis of ligand-binding to kringle 4 and 5 fragmebtsfrom human plasminogen. Thromb. Res. 1989. v.53. №3. P.243-252.
5. Matsuka Yu.V., Novokhatny V.V.,Kudinov S.A. Fluorescence spectroscopic analysis of ligand binding to kringle 1+2+3 and kringle 1 fragment from human plasminogen. Eur.J. Biochem. 1990. V.190. p.93-97.
6. Fibrinolysis mechanism and thrombolytic therapy // Ukr Biokhim Zh. – 2000. – Vol.4-5. – P. 90 – 98.
7. Masliuk AF, Kudinov SA. Methods and prospects of development of the affinitive sorbents for plasminogen isolation from human blood plasma Ukr Biokhim Zh. 2003 May-Jun;75(3):33-44.
8. Patalakh I. I., Kudinov S. O. Нemostasys system and proteins of acute phase response in thrombogenic pathologies // Ukr. Biokhim. Zh. - 2008. – 80 (1). – Р. 5-12
The leading scientist, doctor of biological science Makogonenko Eugen Mitrophanovich. Responsible for the chapter ”The investigation of the mechanism of lateral association of fibrin protofibrils (in cooperation with the Dept. of Proteins structure and functions, the head of the Dept., doctor of biological science Lugovskoi Edward Vitalievich).
Some publications:
1. Makogonenko, E., Goldstein, A. L., Bishop, P.D., and Medved, L., (2004) Factor XIIIa incorporates thymosin ?4 preferentially into the fibrin(ogen) ?C-domains. Biochemistry, 43: 10748-10756.
2. Mincheff, M., Zoubak, S., Altanakova, I., Tchakarov, S., Pogribnyy, P., Makogonenko, E., Botev, S., Meryman, H., (2005) Depletion of CD(25+) cells from human T-cell enriched fraction eliminates immunodominance during priming with dendritic cells genetically modified to express a secreted protein. Cancer Gene Ther., 12: 185-197.
3. Mincheff, M., Zoubak, S., Makogonenko, Y. (2006) Immune responses against PSMA after gene-based vaccination for immunotherapy-A: results from immunizations in animals. Cancer Gene Ther. 13: 436-444.
4. Sarafanov, A.G., Makogonenko, E.M., Pechik, I.V., Radtke, K.P., Khrenov, A.V.,Ananyeva, N.M., Strickland, D.K., Saenko, E.L.( 2006) Identification of coagulation factor VIII A2 domain residues forming the binding epitope for low-density
lipoprotein receptor-related protein. Biochemistry, 45: 1829-1840.
5. Makogonenko, E., Ingham, K.C., Medved, L. (2007) Interaction of the fibronectin COOH-terminal Fib-2 regions with fibrin: further characterization and localization of the Fib-2-binding sites. Biochemistry, 46: 5418-5426.
6. Ananyeva, N., Makogonenko, Y., Kouiavskaia, D., Ruiz, J., Limburg, V., Meijer, A., Khrenov A. V., Shima, M., Strickland, D. K., and Saenko E.L.(2008) The binding sites for the VLDL receptor and LRP are shared within coagulation
factor VIII. Blood. Coagul. Fibrinolysis. 19:166-77.
Senior scientist, candidate of Chemistry science Bereznizki Georgi Konstantinovich.Responsible for the chapter “The synthesis of affinity sorbents for obtaining of protein C concentrate under laboratory conditions”.
Some publications:
Bereznitski G.K.
1.Masluk A.F., Kudinov S.A., Bereznitski G.K., Shikhanova N.A., Shakhnin D.B., Prokopova E.V. Photochemical synthesis of bioaffinity sorbent for plasminogen purification. Ukr. Biochem.J.,2002, v.74, 2, p.45-51.
2.Masluk A.F., Kercha S.F., Shakhnin D.B., Bereznitski G.K., Shikhanova N.A., Sopina I.M., Kudinov S.A. Photoinitiated copolymerization in solution of N-vinylpyrrolidone with monomers of vinyl row. Vysokomolekuliarny soed.,A.,2004, v.46,4, p.1-13.
3. Masluk A.F., Bereznitski G.K.,Sopina I.M., Kercha S.F., Shakhnin D.B., Kudinov S.A. Immobilization of lysin on the crossed granulated copolymer(matrix) of affinity sorbent. Dopovidi Naz.Akad.Nauk. Ukr.,2004,8,p.146-152.
Acting senior scientist, candidate of Biology science Patalakh Iryna Ivanivna. Responsible for the chapters ”The investigation of fibrinolysis mechanism at normal state and during acute phase of inflammation”,“The investigation of the fibrinolysis system during the change of blood redox state ”, “The experimental prove of the possibility of correction of protein C dependant thrombogenic states by the adoption of protein C preparations”.
Some publications:
1. Patalakh I. I., Shtemenko N.I., Baydal O.P.Rhenium cluster compounds as POL regulators in human RBC // Metal ions in biology and medіcinе, 2002, vol. 7. - Р. 489.- 491
2. Patalakh I. I., Zelenyuk M.A., Lemeshko I.V. Free radical process in erythropoesis regulation // Bulletin of the University of Kiev. – 2005. – Vol. 45. – P. 23-25
3. Patalakh I. I. Redox Regulation of Cellular Signalling // Bulletin of the University of Kiev. – 2005. – Vol. 46. – P. 64-67.
4. Patalakh I.I., Lutai M.I., Gavrylenko T.I., Lomakovsky O.M., Kornilina O.M., Shkrabak M.O., Kudinov S.O. Activity of the fibrinolytic system in patients with stable angina pectoris with signs of inflammation // Lab.diagnostics. – 2007. – V.4(42). – P. 14-19.
5. Patalakh I.I., Roka-Moya Ya.M. Method for estimation of blood plasma and serum redox state // Lab.diagnostics. – 2008. – V.1(43). – P. 34-39.
6. Patalakh I. I., Kudinov S. O. Нemostasys system and proteins of acute phase response in thrombogenic pathologies // Ukr. Biokhim. Zh. – 2008. – 80 (1). – Р. 5-12
Acting senior scientist, candidate of Biology science Zhernosekov Dmytro Danilovich. Responsible for the chapter :
“The elaboration of the methods for obtaining of protein C concentrate and other proteins to create preparations for protein C dependent thrombogenic state”,“The elaboration of the methods for obtaining of protein C concentrate and the
detection of protein C activity under different experimental conditions.”
Some publications:
1. Zhernosekov D.D. The role of adhesive proteins in the process of normal and pathological thrombus formation // Lab.diagnostics. – 2007. – # 2(40). – P.72-76.
2. Zhernosekov D.D. Adhesive proteins in inflammation process. //Biopolymers and cell. – 2007. – V.23, 6. – P.483-487
3. Zhernosekov D.D.Cell adhesion molecules of nervous tissue at normal and pathological state. – Vydavniztvo Dnipropetrovsk National university. – 1998. – 80 pages.
